● Secretary
   Ms. Misia Wu
   email: misia@gate.sinica.edu.tw
   Phone: (02) 2787-1306

● NRPB Program PI and Coordinator
   Dr. Ying-Ta Wu
   email: ywu@gate.sinica.edu.tw
   Phone: (02) 2787-1237

● Compound collection and development
   Dr. Shi-Shan Mao
   email: sshanmao@gate.sinica.edu.tw
   Phone: (02) 2787-1303

● Assay Development
   Dr. T-J Rachel Cheng
   email: tingjenc@gate.sinica.edu.tw
   Phone: (02) 2787-1267

● HTS and Cheminformatics
   Dr. Ying-Ta Wu
   email: ywu@gate.sinica.edu.tw
   Phone: (02) 2787-1237

● Medicinal Chemistry
   Dr. Jim-Ming Fang
   email: jmfang@ntu.edu.tw
   Phnoe: (02) 2787-1266
              (02) 3366-1663


Q1:Who can have access to uHTS services?
A1: Anyone having a project approved and supported by NSC or Other agencies can apply for uHTS. This program is supported by NRPB, so we give service priority to the research projects of NRPB.

Q2 : How to arrange for a uHTS assay?
A2: As a new user, you need to complete an account application first. Please have your project information ready when working on both the Account and Project application forms. Once approved, you will be granted access to Project and Assy Managements, where you can add new project and new assay requests. You might have several assays in one project.
Note: A project is a titled research subject; an assay is one of the jobs executed in the project.

Q3: What is the status of your compound? what is the final DMSO concentration?
A3: Our compounds are solubilized in 100% DMSO and reformated to be 1 mM stock in online 1536-well plate.

Assay Development and Validation
The uHTS facility is proficient at helping research investigators automate assays to screen for the identification and investigation of new compounds for basic research and pharmacological discovery.  Through  the advanced, HTS-compatible instruments, the HTS facility supports a wide variety of detection modalities for assay measurements including: absorbance, fluorescence, time-resolved fluorescence, fluorescence polarization, fluorescence resonance energy transfer (FRET), time-resolved FRET, and luminescence. Cell-based and chemical assays in experimental systems that have been used in prior models have included but are not limited to; recombinant proteins, membrane fractions, cellular isolates, cell lysates, and cell lines

A project begins with completion of an Intake Form. Once received, a HTS staff will schedule a consultation to discuss the adaptation and implementation of the assay to the HTS facility. Typically, assays are validated manually by individual laboratories and evaluated to determine the level of automation and instrumentation needed for HTS services. Once the assay is validated with positive and negative controls and the robustness of the assay (Z-factor/Z’) is determined the assay is ready to be automated. During validation of the automation, manual steps are replaced systematically with instruments to ensure accuracy and precision/fidelity of the assay as observed in the manual process.  For example, the Velocity Bravo liquid handler, t can aspirate liquid from an entire plate, wash it, and add a solution to a plate very precisely in a matter of minutes. After determining the sequence of events and instrumentation for the assay, a robotic sequence is created to integrate these assay procedures into an automated fashion.

Software determines the amount of time each sample will take during individual movements of the robot and the composite time for all samples to be assayed. Testing of the schedule begins with running the schedules for individual instruments, followed by testing of all instruments without reagents, and finally, testing of all instruments with water. Next, an experiment is run using all instruments and robotics integrated by the schedule with experimental controls. After the biological procedure and the instrumentation are validated, the assay is ready for compound screening. The growing library available for screening includes over 160,000 compounds.

A large number of data can be generated by HTS and our specialized HTS informatics staff can assist with custom-made software to help assimilate, analyze and reduce data to appropriate specifications for hit picking, follow-up analyses, and hit validation.
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