● Secretary
   Ms. Misia Wu
   email: misia@gate.sinica.edu.tw
   Phone: (02) 2787-1306

● NRPB Program PI and Coordinator
   Dr. Ying-Ta Wu
   email: ywu@gate.sinica.edu.tw
   Phone: (02) 2787-1237

● Compound collection and development
   Dr. Shi-Shan Mao
   email: sshanmao@gate.sinica.edu.tw
   Phone: (02) 2787-1303

● Assay Development
   Dr. T-J Rachel Cheng
   email: tingjenc@gate.sinica.edu.tw
   Phone: (02) 2787-1267

● HTS and Cheminformatics
   Dr. Ying-Ta Wu
   email: ywu@gate.sinica.edu.tw
   Phone: (02) 2787-1237

● Medicinal Chemistry
   Dr. Jim-Ming Fang
   email: jmfang@ntu.edu.tw
   Phnoe: (02) 2787-1266
              (02) 3366-1663

 
 
 

Q1:Who can have access to uHTS services?
A1: Anyone having a project approved and supported by NSC or Other agencies can apply for uHTS. This program is supported by NRPB, so we give service priority to the research projects of NRPB.

Q2 : How to arrange for a uHTS assay?
A2: As a new user, you need to fill in an account application first. Please have your project information ready when working on both the Account and Project application forms. Once approved, you will be granted access to Project and Assy Managements, where you can add new project and new assay requests. You might have several assays in one project.
Note: A project is a titled research subject; an assay is one of the jobs executed in the project.

Q3: What is the status of your compound? what is the final DMSO concentration?
A3: Our compounds are solubilized in 100% DMSO and reformated to be 1 mM stock in online 1536-well plate.

 
 
 
Assay Development
 
 
Assay Formats Screening Capabilities Biologicial Expertise
Fluorescence Intensity
Fluorescence Polarization
TRF
FRET
Luminescence
Absorbance
Cell-based
Enzyme activity

 
Receptor-ligand interaction
Protein-protein interactions
Enzyme Assays
Reporter Assays
Viability Assays
 
The multi-well plate assays are often developed with 96- or 384-well plates in the laboratories. The assays needs to be transferred to 1536-well plate-compatible assays in our facility. The goal of assay development focuses on miniaturizing the assays for 1536-well plates and on the delivery of robust and well validated assays for HTS.
 
Once a high-throughput screening request is approved by the review committee, the core will ask the project PIs to submit an assay questionnaire (provide a hyperlink for document download) for the assays intended for HTS. Based on the questionnaire, the core would adapt currently available protocols to a 1536-well plate format. The assays will be transferred for screening after the assays performed in 1536-well plates can produce readouts with Z' factor > 0.5 and %CV < 15%.
 
Hit confirmation
 
After hits are identified from a high throughput screen, the hits are confirmed and evaluated using the following methods:
 
1) Re-testing: Compounds that were found active against the selected target are re-tested using the same assay conditions used during the HTS.
   
2) Dose response curve generation: Several compound concentrations are tested using the same assay, an IC50 or EC50 value is then generated.
 
Further selectivity and secondary screening will be performed to confirm the identified hits through further collaboration.
 
   
 
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